Abstract
The determination of drug concentration in the serum by laser immunochemical system (i-PiT) is based on the inhibition of immunoprecipitation. To study the precision and accuracy of i-PiT method using a new reagent in determination of valproic acid (VPA) concentration, we used patients' sera undergoing VPA therapy. The precision of i-PiT method applied to low, medium and high VPA level specimens, expressed as coefficient of variation, was less than 7.0 % of within-day and 7.8% of between-run. The mean recovery of 10, 50 and 100 μg/ ml VPA spiked sera was 100.3%. Moreover, to study the accuracy of i-PiT method, VPA concentration in 46 patients' sera was determined by i-PiT and EMIT methods. The correlation coefficient and the regression line were 0.995 and y= 0.996x+ 0.329 (y: i-PiT, x: EMIT), respectively.In terms of precision and accuracy, the new reagent for VPA determination by i-PiT method may be considered as one of the useful reagents for the determination of serum drug concentration in hospital labolatories.
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