Abstract
The duration for which blood used in transfusion is acceptable is an important factor. The validity of blood, conserved and kept at 4°C, has been established by ATP measurement (mol l −1) using a rapid firefly bioluminescence method. ATP is extracted from the blood cells with trichloroacetic acid, diluted with Tris buffer (pH 7.75). Samples conserved with CPDA-1 and ACD were compared. Blood stabilised with CPDA-1 was valid for transfusion until the 33rd day, that stabilised with ACD until the 21st day.
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