Abstract
Objective To establish a method for quantitative detection of urine iodine by catalytic spectrophotometry using discrete chemical analyzer. Methods After digestion of urine samples, discrete chemical analyzer was used to control the reaction temperature and time, determine the iodine content, which replaced the urine iodine arsenic cerium catalytic spectrophotometry (standard method) after sample digestion in the testing process, and the linear range, detection limit, precision and accuracy of the method were tested. The method was used to detect the urine iodine and the results were compared with the standard method. Results The urinary iodine was in a linear range of 0-1 000 μg/L, correlation coefficient r=- 0.999 7. The detection limit of the method was 4.5 μg/L. Precision: the relative standard deviation (RSD) of urine iodine samples with high, medium, and low concentrations were all 0.05). Conclusion Catalytic spectrophotometry using discrete chemical analyzer is succeessfully established, this method is simple, rapid, accurate and easy to operate. Key words: Urine; Iodine; Discrete chemistry analyzer; Catalytic photometry
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