Abstract
A protein of low molecular weight (MW 12, 000) was detected in the urine of a rabbit given cadmium for 17 months, by polyacrylamide gel electro-phoresis in the presence of sodium dodecyl sulfate. This protein was identified as β2-microglobulin by electrosyneresis using standard β2-microglobulin, a urine sample and anti rabbit β2-microglobulin mouse serum. The urinary concentration of β2-microglobulin in the rabbit fed a diet containing cadmium for 17 months was estimated to be 8-40 mg/l by staining the gel with silver and to be 10-20 mg/l (3.6-7.2 mg/day) by electrosyneresis.
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