Determination of uric acid in human urine and serum by capillary electrophoresis with chemiluminescence detection

Abstract

A simple and sensitive method based on capillary electrophoresis (CE) with chemiluminescence (CL) detection has been developed for the determination of uric acid (UA). The sensitive detection was based on the enhancement effect of UA on the CL reaction between luminol and potassium ferricyanide (K 3[Fe(CN) 6]) in alkaline solution. A laboratory-built reaction flow cell and a photon counter were deployed for the CL detection. Experimental conditions for CL detection were studied in detail to achieve a maximum assay sensitivity. Optimal conditions were found to be 1.0 × 10 −4 M luminol added to the CE running buffer and 1.0 × 10 −4 M K 3[Fe(CN) 6] in 0.2 M NaOH solution introduced postcolumn. The proposed CE–CL assay showed good repeatability (relative standard deviation [RSD] = 3.5%, n = 11) and a detection limit of 3.5 × 10 −7 M UA (signal/noise ratio [S/N] = 3). A linear calibration curve ranging from 6.0 × 10 −7 to 3.0 × 10 −5 M UA was obtained. The method was evaluated by quantifying UA in human urine and serum samples with satisfactory assay results.