Determination of ultra trace amounts of metronidazole by 3-phenyl-N-[4-(10,15,20-triphenyl-porphyrin-5-yl)-phenyl]- acrylamide as the fluorescence spectral probe in CTAB microemulsion.

Abstract

In this work, 3-Phenyl-N-[4-(10,15,20-triphenyl-porphyrin-5-yl)-phenyl]- acrylamide (TPPCA) was synthesized for the determination of metronidazole (MTZ). It was found that the type of fluorescence quenching was static quenching determined by Stern-Volmer plot and UV absorption spectroscopy, and thermodynamic related parameters were also obtained. Furthermore, the corresponding measurement conditions: the acidity of the system, the type of surfactant, the concentration of TPPCA and the sequence of reagent addition were optimized. Under the optimal experimental conditions, the linear range of MTZ was determined to be 0.01-0.20 μg mL-1, and the limit of detection (LOD) was 0.004 μg mL-1. Importantly, this report provides a simple, fast, and sensitive probe for the determination of MTZ in pharmaceutical practice.