Abstract
In this study, N-(4-aminobutyl)-N-ethylisoluminol (ABEI) was used as an energy donor, while sodium fluorescein was used as an enhancer and energy acceptor, which resulted in it producing resonance energy transfer and greatly increasing the strength of chemiluminiscence (CL). When horseradish peroxidase (HRP) is added, hydrogen peroxide (H2O2) will quickly separate into hydroxyl radicals (·OH) and superoxide ions (O2·−). If tyrosine (Tyr) is present in the system, the hydroxyl group on the benzene ring of Tyr robs ·OH and O2·− in the CL system, thereby reducing the intensity of CL. Based on this phenomenon, a luminescence system of ABEI and sodium fluorescein system was established to detect Tyr for the first time. This method has an ultra-low detection limit and a wide linear range, and is cheap and easy to operate. Under various optimal conditions, the linear range is from 3.0×10−8 to 3.0×10−5 mol/L, and the limit of detection is 2.4×10−8 mol/L. It has been successfully used in the detection of dairy products with satisfactory results.
Highlights
Chemiluminiscence (CL) refers to the light emitted during chemical reactions
When only sodium fluorescein is added, there is no change in intensity
The effect of sodium fluorescein concentration on energy transfer is observed in this figure
Summary
Chemiluminiscence (CL) refers to the light emitted during chemical reactions (usually in the visible and nearinfrared regions). Tyr can snatch reactive oxygen radicals such as hydroxyl radicals (·OH) and superoxide ions (O2·−) produced by hydrogen peroxide (H2O2) after being oxidized under weakly alkaline conditions, thereby reducing the CL intensity and showing a wider linearity and a lower detection limit. The effect of sodium fluorescein concentration on energy transfer is observed in this figure.
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