Abstract
Plasma protein pools are often virus-inactivated by the solvent–detergent method, using tri- n-butyl phosphate and Triton X-100, followed by removal and determination of these compounds. We used reversed-phase high-performance liquid chromatography for the determination of Triton X-100 in coagulation factor VIII and factor IX products, Octonativ-M and Nanotiv, respectively (Pharmacia, Stockholm, Sweden). The chromatographic system included a C 18 silica column and a linear acetonitrile gradient. The advantage of this method is the low detection limit (0.3 μg/ml) combined with detection at 280 nm, which gives a more stable baseline and has less interference from other compounds. As compared to other methods, where shorter wavelengths are used.
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