Determination of triglycerides in human serum by near-infrared diffuse reflectance spectroscopy using silver mirror as a substrate

Abstract

Near infrared diffuse reflectance spectroscopy (NIRDRS) has gained wide attention due to its convenience for rapid quantitative analysis of complex samples. A method for rapid analysis of triglycerides in human serum using NIRDRS with silver mirror as the substrate is developed. Due to the even and high reflectance of the silver mirror, the spectral response is enhanced and the background interference is reduced. Furthermore, both linear and nonlinear modeling strategies were investigated adopting the partial least squares (PLS) and least squares support vector regression (LS-SVR), continuous wavelet transform (CWT) was used for spectral preprocessing, and variable selection was tried using Monte Carlo uninformative variable elimination (MC-UVE), randomization test (RT) and competitive adaptive reweighted sampling (CARS) for optimization the models. The results show that the determination coefficient (R) between the predicted and reference concentration is 0.9624 and the root mean squared error of prediction (RMSEP) is 0.21. The maximum deviation of the prediction results is as low as 0.473mmol/L. The proposed method may provide an alternative method for routine analysis of serum triglycerides in clinical applications.