Abstract
A TLC-FID method to determine four glyceride types (S3, S2U, SU2, and U3) of fats was developed. Dipalmito-olein, palmito-diolein and triolein were quantitatively oxidised to the corresponding azelaoglycerides with KMnO4, but not tripalmitin. These azelaoglycerides and squalane added as an internal standard were separated on silica-gel TLC and determined with FID. Very good agreement between the determined and calculated compositions was obtained for some synthetic triglyceride mixtures with less polyenoic acid, but not for those with much polyenoic acid. The glyceride type composition of some natural oils and fats with less polyenoic and hydrogenated fats was determined by this method.
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