Abstract
A method for immunonepholometric determination of transcortin has been developed. It is based upon a polyethylenglycol (PEG) enhanced immunonephelometric response using a commercial transcortin antiserum in the assay. It was found that pretreatment of the serum samples with 10% (w/v) PEG 6000 was a necessity in order to obtain satisfactory low blank values. The immunonephelometric assay showed a good correlation with transcortin concentrations as calculated from cortisol binding experiments ( r = 0.9501, n = 26). The intraassay coefficient of variation for a standard serum was found to be 3.2% ( n = 50) and for a pregnancy serum pool 2.6% ( n = 50). The interassay coefficient of variation in ten consecutive analyses of thirteen samples was found to be 7.4%. The sensitivity allows detection of transcortin in 5 μl of serum. The average concentration in male serum was 35 mg/l ( n = 55), in female serum 36 mg/l ( n = 55) and in serum from third trimester pregnant women 81 mg/l ( n = 30) which agrees with the results in previously published reports.
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