Abstract
A new catalytic kinetic method based on the decolorization reaction of rhodamine B has been established for the determination of copper in serum. In the presence of copper, rhodamine B can be oxidized by hydrogen peroxide in the NH3–NH4F medium. After each serum sample was digested with strong acid, the catalytic reaction was carried out in NH3–NH4F buffer (pH9.0) at 100°C for 17 min (1.0 ml buffer, 2.0 ml rhodamine B, 1.2 ml H2O2for a 25 ml reaction mixture). Copper concentration and logA0/Aare linearly related over the concentration range of 20–140 ng/25 ml (r= 0.9999,y= 0.003387x− 0.05969). Repeated assay of a mixture normal serum sample gave CVs of 5.2% (n= 14). The mean recoveries were 98.3%, 101.2%, and 100.2% for three different serum samples. Ca, Mg, Fe, Zn, Na, K, Cl, P, and other elements in serum did not interfere with the determination. The concentrations of copper were 75–170 μg/100ml in serum of 30 normal volunteers; the mean concentration was 115.4 μg/100ml.
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