7-days of FREE Audio papers, translation & more with Prime
7-days of FREE Prime access
7-days of FREE Audio papers, translation & more with Prime
7-days of FREE Prime access
https://doi.org/10.1248/jhs1956.21.204
Copy DOIJournal: Eisei kagaku | Publication Date: Jan 1, 1975 |
License type: free |
Atomic absorption spectroscopic method for the determination of ppb levels of chromium in human urine was investigated by using the technique of a carbon tube atomizer to provide a simple and rapid means for the quantitative determination of total chromium. In order to establish the optimum conditions for the determination of chromium in a sample, various factors such as atomization efficiency including ashing or drying conditions, effect of acids, and matrices were studied. Under the optimum conditions established, the detection limit of the method for S/N = 2 was evaluated as 2×10-11 g and the linearlity of the absobance vs. concentration was good up to 3 ng of chromium. The precision was estimated to be 2.65% (relative standard deviation) from the results of 10 injections of 50 μl of synthetic urine solution containing 20 ppb of chromium, and the recovery of chromium added to synthetic and human urine samples before wet ashing was in the renge of 95-104%. Interference by matrices such as salts and organics in the samples could be eliminated by this technique of double atomization. Chromium concentration in urine from 13 normal subjects ranged from 0.4 to 26.4 ng/ml, which is similar to recent values obtained by several workers.
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.