Abstract
A simple and selective method for the determination of trace amounts of ABS has been developed. It was previously reported that the complexes of Methylene Blue (MB) with methylene blue active substances (MBAS) dissociated into MB and MBAS on silicagel thin-layer chromatography. Based on this phenomenon, ABS contained in MB-MBAS complex could be determined by high performance liquid chromatography (HPLC) under the same conditions as previously reported. The methylene blue active substances in samples were extracted with chloroform in a similar way to MB spectrophotometric method, then concentrated, dissolved in methanol and determined by HPLC. Good recovery and reproducibility were obtained on the determination of ABS in river and waste water. Further, the measured values of ABS agreed well with those obtained by the previously reported method. Therefore, this simple method is applicable to the determination of trace amounts of ABS in river and waste water.
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