Abstract
Aromatic amine and azo compounds are prevalent in the manufacture of common dyestuffs. The toxic actions of these substances are believed to be the result of metabolic activation and formation of labile products. Incubation techniques utilizing the liver microsomal fraction are a common method for studying the formation of these reactive intermediates. This study describes the use of liquid chromatography/electrochemistry (LCEC) for the determination of 4-nitroaniline, 2-amino-5-nitrophenol, and the labile N-hydroxy-4-nitroaniline in microsomal incubations. No prior extraction, preconcentration, or derivatization steps are necessary for the determinations which can be accomplished by a direct injection of the incubation. The dual-electrode detector in the parallel-adjacent configuration is used to confirm peak identity assignments. The series mode of this detector permits determination of 4-nitroaniline and its metabolites as products of the commonly used textile dye, Disperse Orange 3, in incubation media. Detection limits for the compounds of interest are all in the subpicomole range.
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