Abstract
Mammalian body represents the sum of 2 major components: the body fat which varies widely from individual to individual, and fat-free lean mass (FFLM), which is of relatively constant weight in a healthy individual and is of relatively constant composition. Since adipose tissues are metabolically active, knowledge of total body fat could add significance to studies in fat metabolism. Measuring body fat can provide a means of studying the water content of fat-free lean mass (FFLM), under conditions of abnormal hydration or nutrition, and the changes that may occur with age. Lesser and co-workers<sup>1</sup>report on the use of cyclopropane, a relatively safe anesthetic gas and at the same time highly fat-soluble, for the determination of total body fat in an experiment in a closed circuit. The method of study was as follows: A circular rubber diaphragm with a central aperture somewhat less than collar size was slipped over
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