Abstract
Despite its importance for human health, the capacity of the antioxidative defense system (AOS) is still not included among regular biochemical analyses. In this study, we estimated the blood plasma level of the total antioxidant status (TAS) as an appropriate parameter for AOS monitoring with the aim of including it among other biochemical parameters in the regular control of health of the working population. We have applied a method developed by Randox Laboratories (England) that can be routinely performed on automatic chemical analyzers. The TAS level, in correlation with corresponding biochemical parameters, could contribute to better evaluation of the health of individuals and the working population.
Highlights
Production of reactive species, mostly of oxygen (ROS), is an integral part of every aerobic metabolism
Measuring of blood plasma samples for total antioxidant status (TAS) was performed on a Daytona (RX) automatic chemical analyzer using the Randox TAS kit assay with Trolox as a standard
After comparing several methods of assessing the total antioxidant capacity of human serum or plasma, we chose a method based on estimation of blood TAS with Trolox as an equivalent standard (Miller et al, 1993; Rice-Evans and Miller, 1994) because it can be used with an automatic chemical analyzer as a micromethod and has standardization established by a world-renowned laboratory (Randox)
Summary
Production of reactive species, mostly of oxygen (ROS), is an integral part of every aerobic metabolism. Because of the high potential of ROS, especially very reactive free radicals (FR), to damage vital biological systems by forming oxidatively modified biomolecules (proteins, lipids, nuclei acids) that disturb their relatioships and organization (Slater, 1987; Halliwell and Gutteridge, 1989; Floyd and Carney, 1992), living organisms have been forced to develop an antioxidative defense system (AOS). A balance exists between production of ROS and AOS activity. Many factors disturb this equilibrium, thereby reducing the organism’s defense capacity and allowing uncontrolled oxidation of biomolecules. Such factors include exposure of the organism to environmental toxicants, hazardous chemicals, and heavy metals; ionizing radiation; miscellaneous xenobiotics; and endogenous pathological metabolic changes.
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