Abstract
Original coulometric method for total antioxidant capacity (TAC) determination based on the reaction of plasma antioxidants with electrogenerated bromine is developed. TAC is expressed in units of quantity of electricity (Coulombs) spent for titration per a liter of plasma. Coulomb is the universal unit of the TAC measurements because one can express TAC in units of different antioxidants using Faraday constant and the number of electrons involved in the reaction between antioxidant and electrogenerated bromine.The TAC in the plasma being determined for patients with different types of lung diseases is significantly lower than the one for control group. The data obtained by coulometry correlate with results of voltammetric determination based on oxidation of plasma on the glassy carbon electrode (Y=a + b x, where a= -6,7 ± 0,5, b=1,07 ± 0,04, R =0,9934). So, the proposed method could be recommended for clinical laboratories for estimation of antioxidant status and its subsequent correction using additional antioxidant therapy.
Highlights
The current scientific literature is replete with data concerning oxidative stress in biological systems and the role of various antioxidants both in physiological states such as growth and aging, or in pathological conditions as atherosclerosis, neurogenerative and inflammatory diseases and cancer.Ziyatdinova, Budnikov & Pogorel’tzevOxidative stress may be defined as a violation in the balance between oxidants and reductants in living organism
total antioxidant capacity (TAC) is expressed in units of quantity of electricity (Coulombs) which is spent for titration on one liter of plasma
TAC determination is based on the reaction of plasma antioxidants with the electrogenerated bromine as active species
Summary
The current scientific literature is replete with data concerning oxidative stress in biological systems and the role of various antioxidants both in physiological states such as growth and aging, or in pathological conditions as atherosclerosis, neurogenerative and inflammatory diseases and cancer. For assessing endogenous antioxidant capacity, most studies have examined the concentrations of antioxidants (vitamin E, vitamin C, carotenoids, folate, sulfur containing amino acids) in plasma and cells and the cellular activities of antioxidant enzymes (glutathione reductase, superoxide dismutase, catalase and glutathione peroxidase). The electron spin resonance technique is suitable for detecting free radicals in solution chemistry, it has limited application to biological tissues owing to their usually high content of water. This problem can be overcome by the use of the spin trapping technique, which involves the conversion of highly reactive free radicals to relatively inert radicals, followed by electron spin resonance analysis [13]. The aim of work presented is development of coulometric titration for the TAC analysis in biological objects
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