Abstract
The cyclic voltammetry (CV) was used for the measurement of the plasma total antioxidant capacity from two types of patients. The first one consisted of 29 volunteers (men aged 18-21 years) who were administered placebo or silymarin at a dose of 858 mg/day. After two months of silymarine administration, CV revealed a statistically significant increase in total antioxidant capacity compared to placebo. No statistically significant changes in TBARS, SH-groups, creatininin, urea, and uric acid concentrations were found. The second group under study comprised 49 patients with chronic renal disease during dialysis therapy. After dialysis, CV revealed a decrease of total antioxidant capacity in the plasma, which was equivalent to a decrease in creatinine, urea and uric acid. CV was performed using a system consisting of a working glassy carbon electrode, an auxiliary platinum electrode, and a reference saturated calomel electrode; a linear change of voltage of 200 mV/s was applied. CV is a simple and relatively reliable method for assessment of body antioxidant status. It is also time and cost effective.
Highlights
Free radicals are highly reactive molecules generated by biochemical redox reactions that occur as a part of normal cell metabolism and in the course of free radical-mediated diseases such as diabetes mellitus, cancer, rheumatoid arthritis, renal, cardiovascular, inflammatory, infectious and neurological diseases[1]
The depletion of Total antioxidant capacity (TAC) induced by oxidative stress is eliminated by release of stock organ antioxidants, mainly from liver and adipose tissue and the induction or activation of antioxidant enzymes
A significant increase of total antioxidant capacity really occurs after supplementation with vitamins C, E, and ß-carotene[5] and phenolics of green and black tea[6]
Summary
Free radicals are highly reactive molecules generated by biochemical redox reactions that occur as a part of normal cell metabolism and in the course of free radical-mediated diseases such as diabetes mellitus, cancer, rheumatoid arthritis, renal, cardiovascular, inflammatory, infectious and neurological diseases[1]. Determination of TAC is based on the evaluation of a total reduction effect of individual LMW antioxidants, of either a hydrophilic or a hydrophobic character. It provides information about antioxidant types and their concentration without exact qualitative differentiation. Measurement is based on the ability of antioxidants in the sample to inhibit the oxidative effects of reactive species purposefully generated in the reaction mixture. This method is simple, speedy, inexpensive and robust.
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