Determination of Thiophanate-Methyl and Carbendazim in Rapeseed by Solid–Phase Extraction and Ultra–High Performance Chromatography with Photodiode Array Detection

Abstract

A rapid and reliable ultra-high performance liquid chromatography method using a photodiode array detector with QuEChERS (quick, easy, cheap, effective, rugged, and safe) sample preparation was developed to determine thiophanate-methyl and carbendazim in rapeseed. Samples were extracted with acetonitrile and partitioned with a mixture of NaCl and anhydrous MgSO4. The cleanup step was performed using dispersive solid-phase extraction with anhydrous MgSO4 and primary secondary amine. This step was repeated to improve the results. The limits of detection and quantification of thiophanate-methyl and carbendazim in rapeseed were 15 and 50 μg kg−1, respectively. The recoveries of thiophanate-methyl and carbendazim were from 77.44–100.9%, with relative standard deviations between 2.20% and 6.62%. The method was employed to determine thiophanate-methyl and carbendazim in rapeseed. This study has established a novel method and a safety evaluation system for the analytes in rapeseed.