Abstract
An isotope dilution method using Cl/sup 36/ is described in which the true value of the total chloride content of a sample of biological fluid or tissue can be measured with a precision of plus or minus 0.8% (relative standard deviation) for amounts of 150 to 250 mu equiv of chloride. The method involves complete isotopic exchange of stable with added radioactive chloride in a solution formed by hot alkaline digestion of the sample, and determination of the correct value of the diluted specific activity in purified solutions obtained by successive stages of alkaline dry ashing, oxidationreduction, and distillation. Radioassay was performed by infinite thickness liquid counting, and chemical assay was by automatic coulometric-amperometric titration with siiver ion. The true chloride contents of tissues of normal adult rats, dogs, and frogs were lower than most values previously reported, particularly for muscle and liver. The isotope dilution method described can serve as a standard of reference for the evaluation and validation of simpler methods of chloride measurement in biological materials. (auth)
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