Determination of the Total Phenolic and Flavonoid in Various Extracts of Adiantum capillus veneris Linn, as well as their Radical Scavenging Activity

Yusra F Layas,Salah N Bugrein,Maraia F. Elmhdwi ,Naema M El Aali,Mohammed F El-Fellah

doi:10.31080/asmi.2018.01.0002

Abstract

Determination of the Total Phenolic and Flavonoid in Various Extracts of Adiantum capillus veneris Linn, as well as their Radical Scavenging Activity

Highlights

The knowledge about use of medicinal plants has been accrued through centuries time and such plants are still valued even today, synthetics, antibiotics etc. have attained greater prominence in modern medicine
The antioxidant activity of A. capillus veneris L. was confirmed for all extracts with the highest activity for the water extract initial concentration of DPPH• by 50% (IC50) = 2.7, this assay was supported with reducing power assay, in which both methanolic extract and water extract exhibited the highest reducing power assay, while the ethyl acetate extract shows the highest total flavonoids content
The present study gave an insight into the effectiveness of different extracts of the plant A. capillus veneris L. as antioxidant in which the antioxidant activity of A. capillus veneris L. was confirmed for all extracts with the highest activity for the water extract IC50 = 2.7, and the lowest for the acetone extract IC50 = 21.51, this assay was supported with reducing power assay, in which both methanolic extract and water extract exhibited the highest values (0.535 ± 0.028 and 0.494 ± 0.044) respectively at 500 μg/ ml, while the ethyl acetate extract gave the lowest value (0.310 ± 0.000) at 500 μg/ml

Summary

Introduction

The knowledge about use of medicinal plants has been accrued through centuries time and such plants are still valued even today, synthetics, antibiotics etc. have attained greater prominence in modern medicine. To 100 of 100, 200, 300, 400, and 500 mg/L of the different extracts 2 ml of de-ionized water were added and mixed with 600 μl of Folin-Cicalteau reagent, the tube was allowed to stand at room temperature for 5 minutes, and 2 ml of 20% sodium carbonate were add and kept at boiling water bath for 1 minute, after cooling the blue color formed measured at 765 nm by Aquarins (CE700) spectrophotometer Cecil instruments [10]. This assay was determined according to the method of Oyaizu (1986). A calibration curve was prepared with quercetin and the results were expressed as mg quercetin equivalents per gram dry weight of sample [12]

Findings

Result and Discussion Antioxidant activity assay

Conclusion