Determination of the total content of polyphenols, flavonoids and the antioxidant capacity of extracts from Viola x wittrockiana flowers

Abstract

Study objectives: were evaluation of the content in bioactive compounds with antioxidant properties of the species Viola x wittrockiana Gams flowers, cultivated in the Bihor region of Romania. Materials and Methods: Viola x wittrockiana Gams flowers were harvested, dried, then prepared for to evaluate the phenolic and flavonoid contents and for determined antioxidant capacity by the following methods: DPPH and FRAP. Results – after determined the total polyphenolic compounds content the concentration found was contained between 2.5-4.52 mg GAE/100 g dry flowers and flavonoids were contained between 46.75-54.49 mg QE/100 g dry flowers garden pansies. Conclusions: strong correlation between the polyphenols and flavonoids content and antioxidant properties which have been evaluated at the extracts obtained from the pansy flowers have shown that the plant has inhibition capacity on DPPH reagent contained between 62.644 – 92.241% and antioxidant capacity which varies between 494.588-889.882 made by FRAP method.