DETERMINATION OF THE SYNERGISTIC EFFECT OF CURCUMIN AND CARNOSIC ACID ON THE PROLIFERATION OF PROSTATE CANCER CELLS

Abstract

Relevance: Cancer is one of the leading causes of death globally. The results of the conducted studies on the energy metabolism of
 cancer cells can contribute to identifying molecular targets in the treatment of cancer. It is known that polyphenols widely distributed in
 plants have an anti-cancer effect based on their well-known antioxidant, anti-inflammatory, antiproliferative, proapoptotic, and antiangiogenic potential.
 Primary prostate cancer treatment methods include surgical resection and chemo- or radiation therapy. In the last decade, polyphenols
 have attracted attention for use in complex prostate cancer chemotherapy. The search for natural compounds increasing the body’s resistance to tumor development and reducing the possibility of tumor recurrence after radiation or chemotherapy and studying their mechanisms of action are relevant directions for further in vivo research. In addition, there is a growing interest in studying practical treatment
 approaches combining drugs with various mechanisms of action.
 The study aimed to identify the synergistic effect of curcumin and carnosic acid on the proliferation of prostate cancer cells.
 Methods: The proliferative activity of cells in culture on tablets, under the influence of polyphenols in various concentrations, was
 evaluated by Alamar blue using a BioTek Synergy plate reader (BD Biosciences, San Jose, CA).
 Results: При In determining the effect of naturally occurring polyphenols on prostate cancer cell proliferation, the effect of curcumin
 was significantly greater than that of carnosic acid. In a higher concentration, their effect on the proliferation of prostate cancer cells was
 higher and stopped cell growth by 30-60%, depending on the concentration and time of exposure.
 Conclusion: A detailed evaluation of the data on inhibition of PC3, Du145 cell growth revealed a clear synergistic interaction between
 curcumin and carnosic acid, which was especially strong at a concentration of curcumin 7 μM in combination with carnosic acid 5 μM