Abstract
The current methods for measurement of free cortisol are hampered by methodological drawbacks including large sample requirement, length of the procedure, and overestimation or underestimation of free cortisol. We report a technique combining ultrafiltration and dialysis, which could largely overcome these drawbacks, for measurement of the per cent free cortisol in plasma in the dog. This is a modification of the method of Hammond et al. for the determination of sex steroids in man. We found a good positive correlation (r s = 0.87) with equilibrium dialysis, which is considered the golden standard in free cortisol measurements. The 2.5th and 97.5th percentiles of values of free cortisol measured in plasma of 40 healthy household dogs were 5–20 nmol/l or 5.1–11.7% of the total cortisol concentration.
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