Abstract
Determinations of hemoglobin oxygen saturation, oxygen tension, whole blood pH, carbon dioxide tension, red cell 2,3-diphosphoglycerate (DPG), and carboxy-hemoglobin concentration were carried out on venous blood from normal human subjects at rest. When the oxygen tension was calculated for the standard conditions of pH = 7.40, Pc2 = 40 mm Hg, red cell DPG = 5.0 mmol/l, and HbCO = 1.0%, it was found that the individual points coin cided with cided with the standard oxygen dissociation curve as obtained by tonometry. The procedure can therefore be used to obtain a quantitative estimation of the oxygen affinity of whole blood in vivo as well as under standard conditions. The precision is such that a deviation from normality of about 2 mm Hg can be detected by a single determination.
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