Determination of the Organophosphorus Pesticide Diclorvos by Enzymatic Flow- Injection Analysis with Conductimetric Detection

S Khan,E Olaf,M P Da Silvae,M M D C Vila,M Tubino,T C Rodrigues,Nicola Pirrone

doi:10.1051/e3sconf/20130109007

Abstract

Brazil is one of the world largest consumers of pesticides. When used properly pesticides help increase production. But when misused they can be extremely detrimental to the environment and to the population, which has led civil society to demand tighter control from environmental agencies and increased the demand for analytical data. In this work, we developed a scanning method for the determination of organophosphorus pesticides using a FIA procedure associated with an enzymatic biosensor based on acetyl cholinesterase. Although immunosensor systems have been proposed as powerful pesticide monitoring tools. The method is based on the generation of acetic acid by the reaction of acetylcholine with the enzyme cholinesterase. Which was chemically immobilized on CPG the acetic acid formed permeate through a poly- (tetrauoroethylene) (PTFE) membrane into a de-ionized water stream. The developed method is extremely simple, sensitive and low cost and selective for inhibitors of this enzyme. For the pesticide paroxon the detection limit was about 10 -18 mol L -1 , the correlation coefficient was 0.9966 and the standard deviation of 0.0403 (n = 6).

Highlights

Brazil is one of the world's largest consumers of pesticides
We developed a scanning method for the determination of organophosphorus pesticides using a FIA procedure associated with an enzymatic biosensor based on acetyl cholinesterase
The proposed method is based on the formation of acetic acid by reacting acetylcholine with acetyl cholinesterase enzyme and the resulting acetic acid permeates through a diffusion membrane, under a stream of deionized water, being detected using conductometry [3]

Summary

Introduction

Brazil is one of the world's largest consumers of pesticides. The insecticides and organophosphorus pesticides (OPs) are widely used in agriculture worldwide [1]. The biosensors working on the basis of enzyme inhibition have been widely used to perform the detection of organophosphorus compounds [2]. The proposed method is based on the formation of acetic acid by reacting acetylcholine with acetyl cholinesterase enzyme and the resulting acetic acid permeates through a diffusion membrane, under a stream of deionized water, being detected using conductometry [3]

Methods and Materials

Result and Discussion

Conclusion