Abstract
In the current study, four types of plants commonly used namely Soybean, chickpea, bean, pea were obtained and screened for urease activity, among this plants, chickpea was chosen with maximum enzymatic activity, and it had the highest productivity of urease enzyme (1243 U/mg protein). Also sodium acetate buffer (0.2 M, pH 5.0) was chosen as a best extraction buffer with specific activity 1460 U/mg protein. The optimum extraction ratio represented by 1:8 (w:v) after 15 min, it was given 1988 U/mg protein. As well as four types of plants include garlic, red onion, green onion and cabbage were used to select the optimum plant material that inhibited urease enzyme. Cabbage was chosen, it had the highest inhibition activity of the enzyme (41%). Also tris buffer (0.2 M, pH 9) was selected as a best extraction buffer of plants inhibitor with inhibition activity 80%. The optimum extraction ratio represented by 1:8 (w:v) after 60 min, it was given 86% enzyme inhibition activity.
Highlights
Urease (EC 3.5.1.5; urea amidohydrolase) is a nickel-dependent enzyme that catalyzes the hydrolysis of urea to ammonia and carbamate; the latter decomposes spontaneously, producing another molecule of ammonia and carbon dioxide (15)
Research has indicated that urease of H. pylori and Proteus mirabilis is located in the cytoplasm in freshly prepared cultures and in the outer membrane in older cultures (11)
This study has focused on monitoring and evaluating the ability of 4 herbs for their possible inhibitory activity against plant urease
Summary
Urease (EC 3.5.1.5; urea amidohydrolase) is a nickel-dependent enzyme that catalyzes the hydrolysis of urea to ammonia and carbamate; the latter decomposes spontaneously, producing another molecule of ammonia and carbon dioxide (15). It’s a key enzyme benefiting bacteria H. pylori and Proteus mirabilis through making its persistence possible in the acidic environment of the stomach and as a result, cause gastrointestinal diseases, in particular gastritis, duodenal, peptic ulcer, and gastric cancer (3). Urease activity leads to other disease like urinary stones, pyelonephritis (24). Urease is produced by a wide variety of plants, fungi, bacteria and invertebrates, enabling them to utilize urea as a nitrogen source (18). Specific inhibition of urease activity has been proposed as a possible strategy to inhibit this microorganism (20). Many naturally occurring compounds found in dietary and medicinal plants, herbs, and fruit extracts have been shown to possess antimicrobial activities (25). This study has focused on monitoring and evaluating the ability of 4 herbs for their possible inhibitory activity against plant urease. Determination the optimum extraction condition for both enzyme and inhibitor from some local plants
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