Determination of the N-Nitroso Compounds in Mouse Following RDX Exposure.

Abstract

Hexahydro-1,3,5-trinitro-1,3,5-triazine, commonly called RDX, is an important explosive, which is widely used in military and civic activities. As it is used, RDX is widely found in many locations and caused soil and water contamination. Many studies show that RDX is toxic to many organisms, including plants, animals, and microbes. RDX causes genetic toxicity and neurotoxicity as well as potential carcinogenesis. Even it is worse that RDX can be biotransformed into other N-nitroso derivatives, such as MNX, DNX, and TNX; these derivatives can be found in both naturally in RDX-contaminated soil and also in the animal GI tracks. To study the potential effect of RDX and its N-nitroso derivatives, this chapter presents a step-by-step method for detect RDX and its N-nitroso derivatives in animal stomach and GI tracts followed RDX exposure by gas chromatography with electron capture detector (GC/ECD). This method can also be used to detect RDX and its N-nitroso derivatives in other tissues and in other animals and plants.