Abstract
In the present research, mosquito fish Gambusia affinis have been exposed to lead chloride during 24, 48, 72 and 96 hours in order to evaluate the lead chloride lethal 50 (LC50) concentration and the Its residue in certain organs of fish. Usage of the EPA computer software based on Finney Probit Analysis method has been statistically tested for the data collected LC50 values of G. affinis if 24, 48, 72 and 96 hours were found to be 59.4, 55.9, 51.1 and 49. 0 mg/L, respectively. LC50 decreased as mean exposure times. 20 fish were placed in each concentration of four sublethal concentrations 20 and 25 mg/L for two acute periods 24 and 96 hours as well as 10 and 5 mg/l for chronic periods 15 and 30 hours. The testes were carried out as three replications, the accumulation of lead in various fish organs was determined by Atomic Absorption Spectrophotometer. The finding revealed that the accumulation of PbCl2 on different organs of G. affinis be time dependent fashion and Pb-content in organs increased significantly time dependent at chronic exposure as compared as acute- exposure.
Highlights
Owing to their toxicity, heavy metal is considered to be environmental contaminants, persistence in the ecosystem and bio accumulative existence [1]
Usage of the EPA computer software based on Finney Probit Analysis method has been statistically tested for the data collected LC50 values of G. affinis if 24, 48, 72 and 96 hours were found to be 59.4, 55.9, 51.1 and 49. 0 mg/L, respectively
LC50 decreased as mean exposure times. 20 fish were placed in each concentration of four sublethal concentrations 20 and 25 mg/L for two acute periods 24 and 96 hours as well as 10 and 5 mg/l for chronic periods 15 and 30 hours
Summary
Heavy metal is considered to be environmental contaminants, persistence in the ecosystem and bio accumulative existence [1]. It is characterized as a metal with density is bigger than 5 g/cm and their atomic weight is 63.5-200.5 g/mol [2]. Lead is a highly poisonous metal, and its routine use has caused excess contamination of the environment and associated health problems in many parts of the world [7]. Fish have been recognized as the main aquatic organisms that accumulate considerable amounts of certain metals exceeding their concentrations in the aquatic ecosystem [8]. The purpose of the present study was to determine the LC50 of lead chloride in acute and chronic treatment of mosquito fish lead accumulation in various G. affinis organs
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