Abstract
The isoelectric points of rat liver mitochondria and mitochondrial membrane fractions determined by two independent methods, cross-partition in sodium salts and isoelectric focusing in Ampholine gradients, are in good agreement. The determined isoelectric points are 5.2–5.4 for whole mitochondria, 5.3 for the inner membrane fraction and 4.8–4.9 for the outer membrane fracton. This work shows that cross-partition which has previously only been applied to proteins can also be used to determine the isoelectric points of cell organelles and membranes. It is suggested that cross-partition in phase systems can be used to study charge and conformational changes in membranes.
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