Abstract
The time dependent uptake of 1-anilino-8-naphthalene sulphonate (ANS) by bovine erythrocytes was studied. Experimental results were compared with theoretical predictions in order to determine the inner erythrocyte membrane surface potential. The value of the inner membrane surface potential under physiological conditions was estimated as −35 mV. In addition, the number of ANS binding sites on the outer surface of the single bovine erythrocyte membrane was determined as 2.2 × 10 6.
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