Determination of the inhibitory activity of some biological extracts agaiast multi rrsistans antibiotic Staphylococcus specis which and isolated from different sources of infechtion

Abstract

The study include 225 specimens that were collected from patients attended Tikrit General Hospital from mid of Septembar to mid December 2016. The specimens included the swab from: burn, wound, otitis media ,pharyngitis, and urine The specimens were incubated on to mannitol salt agar which is considered as selective and differential media for the genus Staphylococcus the results showed that 100 isolate (44,5%) grown on the medium. According to the phenotypic characteristics and tests biochemical they were Staph. aureus ,Staph. Saprophytis , Staph. sciuri, Staph. Xylosus Staph. lentus Staph. haemolyticusi Staph.c aseolytic 
 The ability of isolates to produce nitrate reductase, hemolysin, urease, DNAas were test . The antiboiotics were tested for resistance to 10 antibiotics. Some spceics showed high resistance to PenicillinG (94), Tobramycin (40%), Amikacin (39%), NitroFurantion (38%) Tetracyclin (36%), ,Novobiocin (24%), Trimethoprim (23%), Gentamycin (13%), Chloromphenicol (10%), Imipenem (3%).,
 The inhibitory effect of biological extract of the Staph. species the pylogenetic effect has built of the concentrations of 100,150,250 mg / ml showed an inhibitory effect against types of Staphylococcus bacteria. 
 It was also found that the higher the concentration of the extract, the greater the inhibitory effect.
 The results showed that Staph.aurea bacterial was the most affected by fungal compounds if the highest inhibition dimeter in concentration was 250 mg / ml 26 mm in the case of extract of Trichoderma koningii, whereas the inhibition rate in the same case the concentration was 24 ml. in the case of extract of Trichoderma viride.