Abstract
Ethyl 2,4-decadienoate isomers, important aroma compounds in the Bartlett pear, were investigated. Separation and identification of isomers was performed. Separation of isomers was successful using gas chromatography (GC) with a VOCOL capillary column and high-performance liquid chromatography on a nonpolar reversed-phase column (C 18) with silver ions in mobile phase. Identification of the isomers was made by comparison of their retention times with the authentic ethyl trans-2, cis-4-decadienoate standard, and by NMR spectrometry. For quantitative determination of ethyl 2,4-decadienoate isomers the limit of detection and the interval of linearity for ethyl trans-2, cis-4-decadienoate were determined for both chromatographic methods.
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