Abstract
HYPOXIA INFLUENCE AQUAPORIN 3 (AQP3) AND MIGRATION IN ORAL SQUAMOUS CELL CARCINOMA (OSCC) Tine M. Soland, Camilla Husvik Ulekleiv, Hilde Kanli Galtung, Department of Oral Biology, University of Oslo, Norway Objective: Hypoxic conditions are associated with more aggressive tumours. Aquaporins seem to have key roles in tumour biology and we hypothesise that the expression and effect on tumour characteristics are influenced by AQP3 level regulated by hypoxia in OSCC. Study Design: AQP3-expression in PE/CA-PJ34 and H413 OSCC cell-lines were evaluated by RT-PCR and Western blot. Hypoxia was induced in OSCC cells by 20 hours exposure to 1.0mM CoCl2. RNA and protein were extracted and AQP3 levels were studied by RT-PCR and Western blot. The influence of 1.0mM CoCl2 on wound healing (migration) and proliferation was evaluated. Mean per cent wound closure of groups was compared using t-test for normally distributed data. Results: Preliminary, AQP3 mRNA and protein were detected in OSCC cells. CoCl2 reduced the expression of AQP3 mRNA, wound healing ability and cell proliferation. Conclusions: Reduced AQP3 in hypoxic conditions indicate less aggressive OSCC properties.
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