Determination of the effects of irisin hormone in SKMEL-30 cells

Abstract

Melanocytes, the skin's pigment-producing cells, are the source of the skin cancer known as melanoma. Numerous variables, including as immune system interactions, tumor microenvironment, and genetic alterations, have an impact on the development and behavior of melanoma. The purpose of this study was to ascertain the impact of irisin on melanoma cells. The molecular effects of irisin SKMEL-30 on human melanoma cancer cells were examined for this aim. By using MTT technique, the effects of irisin on cell growth were examined. Real-time polymerase chain reaction was used to examine changes in gene expression level. The concentrations of sialic acid were measured using spectrophotometry. In the investigation, the irisin IC50 value for a 24-hour application was determined to be 30 nM. In comparison to the control group, sialic acid levels in the irisin-treated group of SKMEL-30 cells were significantly lower. In the qRT-PCR investigation, ST8SIA-2, one of the glycosyltransferase genes, increased 12.591-fold in the application group whereas cas8, one of the apoptotic genes, increased 82.481-fold. In conclusion, flow cytometry analyses proved that administration of 30 nM irisin to SKMEL-30 cells influences cell proliferation but does not cause apoptosis. It was shown that sialic acid substitution reduced the proliferative and metastatic potential of SKMEL- 30 cells.