Abstract
The present study deals with the determination of effective component in the hatching media . it reaveled that there is a large variability in the effects of different solutions on the percentage ofhatching . The hatching index of the different solutions showed that bile was most effective component and respond for 55.3% of hatching . The second factor was sodium bicarbonate ( NaHCO3 ) solution 42.83%, trypsin gave 14.0% of hatching and then the pancreatin which gave10.2% . Each of hydrochloric acid and pepsin solution doesn’t affect the percentage of hatching. The component of Artificial Intestinal Solution ( AIS ) is very important for the completion of hatching process , where the absence of each component resulted in a change of the hatching rate . There were significant differences between the percentage of hatching.
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