Abstract
Radioactive cesium ions have been used as indicators for the unequal distribution of electrolytes in two-compartment systems which contain non-diffusible macroions in one of the compartments. This method allows the precise determination of the Donnan ratio under conditions in which binding studies of small molecules to proteins are carried out. The corrections, which can be performed easily, are required for ligands whose affinities to proteins are low, particularly if electrostatic protein-ligand interactions preclude the use of high concentrations of diffusible ions. This is illustrated by the comparison of the Donnan equilibrium on the binding of succinate and of CTP to aspartate transcarbamylase from E. coli.
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