Abstract
We evaluated the H +:Na + coupling ratio of the Na +-H + exchanger present in microvillus membrane vesicles isolated from the rabbit renal cortex. Our approach was to impose transmembrane Na + and H + gradients of varying magnitude and then to measure the net flux of Na + over the subsequent 5-s period. The Na +-H + exchanger was observed to be at equilibrium (i.e. no significant net Na + flux) whenever [Na +] i/[Na +] o was equal to [H +] i/[H +] o. Moreover, under all conditions the magnitude and direction of net Na + flux was independent of changes in the transmembrane electrical potential difference. These results are consistent with a value of 1.0 for the coupling ratio of Na +-H + exchange in renal microvillus membrane vesicles.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Biomembranes
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