Determination of the content of chitosan in chitosan gel and evaluation of its therapeutic effect on chemotherapeutic phlebitis

Abstract

Objective To assay the content of chitosan in a kind of self-manufacture chitosan gel and evaluate its therapeutic effect on chemotherapeutic phlebitis. Methods To assay the content of chitosan in self-manufacture chitosan gel, the ninhydrin colormetric analysis method was employed. And the therapeutic effect of the chitosan gel on chemotherapeutic phlebitis was estimated by the model of phlebitis of rabbit ear vein. Results The content of chitosan in gel was determined by the method of ninhydrin colormetric. The concentration and absorbance of chitosan had good linear relation from 4.0μg/ml to 40.0μg/ml (r=0.9969), the average recovery was 101.80%. The result of the LSD-t test showed that the self-manufacture chitosan gel had no pronounced difference from positive control drug in curing the chemotherapeutic phlebitis (t=0.939, P=0.362). Conclusion The content of the chitosan in chitosan gel was successful determined by the method of ninhydrin colormetric analysis method, and the self-manufacture chitosan gel had the effect in curing or accelerating the recovery process of chemotherapeutic phlebitis. Key words: Chitosan gel; Chitosan; Content analysis; Chemotherapeutic phlebitis; Therapeutic effect