Abstract
The paper presents the results of research on the development of immune biosensor test system for express detection of polyamines in cells of breast cancer. Determination of polyamines was performed by using an analytical device - immune biosensor based on surface plasmon resonance (SPR), where antigen-antibody reaction is performed in real time on the surface of transducer, resulting in formation of immune complexes and recording the shift of resonance angle. It was performed two methods of analysis: direct, when immobilized specific antibodies interacted with appropriate antigens and competitive, when the one part of antigens was conjugated with protein and competed for binding with specific antibodies with free antigens. In relation to direct analysis it was shown the dependence of sensitivity of biosensor response from the concentration of polyamines in range of 10-100 ng /ml. Concerning the competitive method of analysis, the linear region of sensitivity was in range of 0,3-80 ng/ml and 0,01-90 ng/ml for spermine and spermidine respectively.
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