Abstract
Death due to hypovolemic shock may occur when the blood volume lost reaches about one-third of the whole volume contained in an adult human body [1]. Bloodstained clothes and other objects found in a car were sent to our laboratory. The aim was to investigate the identity and the amount of blood present on the clothes and objects in order to determine if the amount of blood present on them was enough to cause death. DNA extractions have been performed from each sample [2]. AmplFSTR Profiler Plus and COfiler kits (Applied Biosystems) have been used for PCR amplifications to determine the entity of bloodstains. Dry weight of blood measurement has been performed using the Strassmann and Ziemke method [3], by which identical surfaces have been cut, both completely stained with blood or totally clean. Weight means for both stained and clean cuttings have been calculated for each piece of clothing. Dry weight of blood contained in spots on clothes per surface unit has been obtained by calculating differences between both values. Total
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