Abstract
Trace levels of oxytetracylcine spiked into commercial milk samples are extracted, cleaned up, and preconcentrated using a C18 solid-phase extraction column. The extract is then analyzed by a high-performance liquid chromatography (HPLC) instrument equipped with a UV detector and a C18 column (150 mm × 4.6 mm × 3.5 μm). An isocratic mobile phase of 0.010 M oxalic acid/acetonitrile/methanol (in 15/2/2 ratio by volume) is employed with an average elution time of 5 min. The method has been successfully used in an upper-class instrumental analysis laboratory course to determine the concentration of oxytetracycline at 50 μg/g in the spiked milk sample. Students’ results show an RSD of 18% and a percent error of −1%.
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