Determination of the Antibacterial Drug Enrofloxacin by Solid-Phase Spectrofluorimetry

Abstract

ABSTRACT A method for the determination of trace amounts of Trovafloxacin has been developed, based on solid-phase spectrofluorimetry (SPF). The relative fluorescence intensity of Trovafloxacin fixed on Sephadex SP C-25 gel was measured directly using a solid-phase attachment after packing the gel beads in a 1 mm silica cell. The wavelengths of excitation and emission were 274 and 420 nm, respectively. Using a sample volume of 500 ml the linear concentration range of application for Trovafloxacin was 7–160 ng ml−1, with a relative standard deviation of 1.2% (for a level of 80 ng ml−1) and a detection limit of 2 ng ml−1. It was applied to the determination of Trovafloxacin in human urine samples. It was validated using HPLC as a reference method. Recovery levels of the method reached 100% in all cases.