Determination of the aglycon moieties of glycosidically bound compounds in Flos Chrysanthemi by GC × GC–TOFMS

Abstract

A credible method for determination of the aglycon moieties of glycosidically bound aroma compounds in Flos Chrysanthemi by comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (GC × GC–TOFMS) has been proposed. The aglycon moieties of glycosidically bound aroma compounds were isolated using methyl-tert-butyl ether (MTBE) extraction following enzymatic hydrolysis. The GC × GC–TOFMS analysis was performed to comprehensively identify different forms of the released aroma components in Flos Chrysanthemi. The result shows that the limit of detection of the released aglycon moieties ranged from 0.3 to 3.1 ng/mL, the recovery of the released 1-octanol was better than 98.3%, and the intra-day and inter-day precisions of this method were 0.2 to 8.9% and 1.3 to 9.1%, respectively. The proposed method was applied to the analysis of four types of Flos Chrysanthemi (Chuju, Boju, Hangju, and Gongju). A total of 60 aglycon moieties of interest were identified in the four types of Flos Chrysanthemi. These aglycones mainly consisted of aliphatic, aromatic, monoterpene, C13-norisoprenoids, and miscellaneous compounds.