Determination of the aflatoxin AFB1 from corn by direct analysis in real time-mass spectrometry (DART-MS)

Abstract

Direct analysis in real time (DART) ionisation coupled to a high-resolution mass spectrometer (MS) was used for screening of aflatoxins from a variety of surfaces and the rapid quantitative analysis of a common form of aflatoxin, AFB1, extracted from corn. Sample preparation procedure and instrument parameter settings were optimised to obtain sensitive and accurate determination of aflatoxin AFB1. 84:16 acetonitrile water extracts of corn were analysed by DART-MS. The lowest calibration level (LCL) for aflatoxin AFB1 was 4 μg kg–1. Quantitative analysis was performed with the use of matrix-matched standards employing the 13C-labelled internal standard for AFB1. DART-MS of spiked corn extracts gave linear response in the range 4–1000 μg kg–1. Good recoveries (94–110%) and repeatabilities (RSD = 0.7–6.9%) were obtained at spiking levels of 20 and 100 μg kg–1 with the use of an isotope dilution technique. Trueness of data obtained for AFB1 in maize by DART-MS was demonstrated by analysis of corn certified reference materials.