Abstract
A double isotope dilution method is presented for the determination of testosterone and andros T-4-ene-3, 17-dione concentration in human plasma consisting of the main following steps: 1/ ether extraction and Florisil column chromatography; 2/ paper chromatography (System 1) for separation of testosterone and androst-4-ene-3, 17-dione; 3/ NaBH 4 reduction of andros T-4-ene-3, 17-dione to testosterone and paper chromatography (System 11); 4/ acetylation of testosterone with C 14-acetic anhydride; 5/ bidimensional thin layer chromatography; 6/ paper chromatography (System 111); 7/ NaBH 4 reduction of testosterone acetate to 3β-hydroxy-androst-4-ene-17β-acetate; 8/ paper chromatography (System 1). After correction for the blank, mean testosterone and androst-4-ene-3,17-dione plasma concentrations were found to be 55 4 and 99 mμg/100 ml for normal adult males and 41 and 180 mμg/100 ml for normal adult females.
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