Abstract
The aim of our present work was the development of a rapid high-performance liquid chromatography method with electrospray ionization and tandem mass spectrometry detection (LC-ESI-MS/MS) for the determination of several corticosteroids in cosmetic products. Corticosteroids are suspected to be illegally added in cosmetic preparations in order to enhance the curative effect against some skin diseases. Sample preparation step consists in a single extraction with acetonitrile followed by centrifugation and filtration. The compounds were separated by reversed-phase chromatography with water and acetonitrile (both with 0.1% formic acid) gradient elution and detected by ESI-MS positive and negative ionization mode. The method was validated at the validation level of 0.1 mg kg−1. Linearity was studied in the 5–250 μg L−1 range and linear coefficients (r2) were all over 0.99. The accuracy and precision of the method were satisfactory. The LOD ranged from 0.085 to 0.109 mg kg−1 and the LOQ from 0.102 to 0.121 mg kg−1. Mean recoveries for all the analytes were within the range 91.9–99.2%. The developed method is sensitive and useful for detection, quantification, and confirmation of these corticosteroids in cosmetic preparations and can be applied in the analysis of the suspected samples under investigation.
Highlights
Corticosteroids are known to be highly effective drugs widely used for the treatment of inflammatory diseases
The aim of our present work was the development of a rapid high-performance liquid chromatography method with electrospray ionization and tandem mass spectrometry detection (LC-electrospray ionization mode (ESI)-MS/MS) for the determination of several corticosteroids in cosmetic products
Side effects and efficacy have to be related to their antiinflammatory propriety, though no active principle shows better risks/benefits ratio compared to the others [1]
Summary
Corticosteroids are known to be highly effective drugs widely used for the treatment of inflammatory diseases In dermatology, they were used for the treatment of skin disorders such as psoriasis, dermatoses, and eczema. They were used for the treatment of skin disorders such as psoriasis, dermatoses, and eczema They reduce inflammation and can temporarily relieve the symptoms of inflammatory skin problems of severe plaque psoriasis. The best method to evaluate the potency of corticosteroids for topical usage is the vasoconstriction test, which allows assessing the vasoconstrictor effect induced by formulations for topical usage in healthy subjects [2] Such test is not quite accurate because it does not consider the treatment period and frequency nor the individual response [3]. Active principles with low potency can be used for a long time and on wide skin surface; the principles with high potency should be used for short time and not applied on sensitive skin areas, such as face and armpits [5]
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