Determination of teicoplanin and ramoplanin residues in aquaculture products by a modified QuEChERS method combined with UPLC-MS/MS

Abstract

A QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method was developed to provide good analytical results for teicoplanin (TEC) and ramoplanin (RAM) residues in aquaticproducts. The analytes was extraction from aquatic product samples using chitosan in the QuEChERS procedure. Compared to conventional adsorbents, chitosan has a relatively good purification ability for the targets.The matrix effects of aquatic products, such as crucian carp, carp, silver carp, tilapia, mandarin fish, turbot, shrimp, sea cucumber and so on, were evaluated by high performance liquid chromatography coupled to triple-quadrupole tandem mass spectrometry. Throughout the validation experiments, the limits of detections and quantifications ranged from 0.4 ∼ 0.8 μg kg−1 and 1.0 ∼ 3.0 μg kg−1, respectively. Calibration curves showed correlation coefficient values higher than 0.998 in range between 1 and 100 μg kg−1. The recoveries ranged from 89.8 % to 110.0 % with relative standard deviation less than 7.2 % in nine typical aquaticproducts. The method shows low matrix effect (-18.5 %∼-2.6 %) for all targets in different aquaticproducts. The proposed methods proved to be reliable, effective and enables rapid determination of large batch samples, indicating its good applicability for detection and monitoring of TEC and RAM residues in aquaticproducts.