Determination of Tea Aroma Precursor Glycosides: An Efficient Approach via Liquid Chromatography-Tandem Mass Spectrometry.

Abstract

Tea aroma components are often stored as glycosidically bound forms in the tea plant (Camellia sinensis). However, the determination of these glycosides in tea samples is far from optimal. In the present study, we developed a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for simultaneous quantification of eight primary aroma glycosides within 10 min. After systematic optimization of multiple reaction monitoring (MRM) parameters, the proposed method was highly sensitive and accurate. Optimization of the method permitted the efficient extraction of aroma glycosides. The developed method was applied to analyze the contents of aroma glycosides in different organs of tea plants, including the bud, leaves, and stem. Contents of aroma glycosides in the harvested 'Shaancha 1' ranged from 36.1 to 40454.4 μg kg-1. Geranyl glucoside and primeveroside mainly accumulated in young leaves, while other glycosides mainly accumulated in mature leaves. The findings document a rapid, reliable, and efficient analysis method. This method will be helpful in elucidating the biosynthesis and biotransformation mechanism of tea aroma glycosides and in promoting the development of the tea industry using advanced technological control approaches during the cultivation of tea plants and tea manufacture.